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1 2 3 "Crystallization and preliminary X-ray analysis of endoglucanase from Pyrococcus horikoshii"

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After electrophoresis, the gels were stained with a particular dye for the substrate using a solution containing 0.125 M Tris-Cl (pH 7.4), 0.5 mM magnesium chloride (MgCl 2 ), 1 mg/mL nicotinamide adenine dinucleotide (NAD), 0.1 mM Li-lactate, 0.25 mg/mL nitro blue tetrazolium, 0.01 M sodium chloride (NaCl), and 0.025 mg/mL PMS

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